Cd19 monoclonal antibody, clone 6D5
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More Files
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Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd19 monoclonal antibody, clone 6D5 (Cat # MAB3226)-FITC and anti-mouse CD3-R-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Cd19.
Immunogen
Native Cd19 from mouse CD19-expressing K562 human erythroleukemia cells.
Host
Rat
Reactivity
Mouse
Specificity
CD19 B-cell differentiation antigen, Mr 95 KDa.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd19 monoclonal antibody, clone 6D5 (Cat # MAB3226)-FITC and anti-mouse CD3-R-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd19
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Publication Reference
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The signaling activity of murine CD19 is regulated during cell development.
Krop I, Shaffer AL, Fearon DT, Schlissel MS.
Journal of Immunology 1996 Jul; 157(1):48.
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Self-renewal of B-1 lymphocytes is dependent on CD19.
Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT.
European Journal of Immunology 1996 Jan; 26(1):238.
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A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors.
Rolink A, ten Boekel E, Melchers F, Fearon DT, Krop I, Andersson J.
The Journal of Experimental Medicine 1996 Jan; 183(1):187.
Application：Flow Cyt, IP, Human, K-562 cellsRAG-2T.
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The signaling activity of murine CD19 is regulated during cell development.
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