Itgb2 monoclonal antibody, clone C71/16
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More Files
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Flow Cytometry
BALB/c splenocytes were stained with Itgb2 monoclonal antibody, clone C71/16 (Cat # MAB3225). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against native Itgb2.
Immunogen
Native Itgb2 from cell membrane of mouse T cell lymphoma BW5147.
Host
Rat
Reactivity
Mouse
Specificity
Common beta2 subunit (Mr 95 KDa) of the LFA-1 integrin complex.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were stained with Itgb2 monoclonal antibody, clone C71/16 (Cat # MAB3225). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Itgb2
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Publication Reference
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Ins and outs of LFA-1.
Lub M, van Kooyk Y, Figdor CG.
Immunology Today 1995 Oct; 16(10):479.
Application：Flow Cyt, Human, Mouse, Monocytes.
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Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1.
Trowbridge IS, Omary MB.
The Journal of Experimental Medicine 1981 Nov; 154(5):1517.
Application：IF, IP, WB-Ce, Mouse, BW5147, M1 cells.
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Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
Springer T, Galfrè G, Secher DS, Milstein C.
European Journal of Immunology 1978 Aug; 8(8):539.
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Ins and outs of LFA-1.
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