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Last updated: 2021/1/24
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PPIF monoclonal antibody, clone AT1F5 

  • Catalog # : MAB3214
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant PPIF.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 30-207 of human PPIF.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Immunohistochemistry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: 293T cell lysate, Lane 2: HepG2 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: PC3 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of human breast cancer tissue were incubated with PPIF monoclonal antibody, clone AT1F5 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells. The cell was stained with PPIF monoclonal antibody, clone AT1F5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • PPIF
  • Gene Alias:
  • CYP3,Cyp-D,FLJ90798,MGC117207
  • Gene Description:
  • peptidylprolyl isomerase F
  • Gene Summary:
  • The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019925,PPIase,cyclophilin 3,cyclophilin D,cyclophilin F,peptidyl-prolyl cis-trans isomerase, mitochondral,rotamase
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