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Last updated: 2021/1/24
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NFATC1 monoclonal antibody, clone AT1C3 

  • Catalog # : MAB3209
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NFATC1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 428-716 of human NFATC1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: 293T cell lysate, Lane 2: NFATC1 Transfected 293T cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells. The cell was stained with NFATC1 monoclonal antibody, clone AT1C3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of Jurkat cells. The cell was stained with NFATC1 monoclonal antibody, clone AT1C3 at 2-5 ug for 1x16cells. (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
  • Application Image
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 4772
  • Gene Name:
  • NFATC1
  • Gene Alias:
  • MGC138448,NF-ATC,NFAT2,NFATc
  • Gene Description:
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
  • Gene Summary:
  • The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq
  • Other Designations:
  • NFAT transcription complex cytosolic component,nuclear factor of activated T-cells, cytosolic component 1
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