Products

Western Blot

Western blot analysis of Lane 1: 293T cell lysate, Lane 2: NFATC1 Transfected 293T cell lysate.

Immunocytochemistry

Immunofluorescence

Immunofluorescence analysis of HeLa cells. The cell was stained with NFATC1 monoclonal antibody, clone AT1C3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of Jurkat cells. The cell was stained with NFATC1 monoclonal antibody, clone AT1C3 at 2-5 ug for 1x1⁶cells. (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Entrez GeneID

4772

Protein Accession#

NP_765978

Gene Name

NFATC1

Gene Alias

MGC138448, NF-ATC, NFAT2, NFATc

Gene Description

nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

Omim ID

600489

Gene Ontology

Hyperlink

Gene Summary

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq

Other Designations

NFAT transcription complex cytosolic component|nuclear factor of activated T-cells, cytosolic component 1