The lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with CFL1 monoclonal antibody, clone AT1C1 (Cat # MAB3208 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM