CFL1 monoclonal antibody, clone AT1C1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: K562 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: LnCap cell lysate, Lane 5: PC3 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CFL1 monoclonal antibody, clone AT1C1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CFL1.
Immunogen
Recombinant protein corresponding to amino acids 1-166 of human CFL1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: K562 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: LnCap cell lysate, Lane 5: PC3 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CFL1 monoclonal antibody, clone AT1C1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — CFL1
Entrez GeneID
1072Protein Accession#
NP_005498Gene Name
CFL1
Gene Alias
CFL
Gene Description
cofilin 1 (non-muscle)
Omim ID
601442Gene Ontology
HyperlinkGene Summary
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Mapping the ADF/cofilin binding site on monomeric actin by competitive cross-linking and peptide array: evidence for a second binding site on monomeric actin.
Mannherz HG, Ballweber E, Galla M, Villard S, Granier C, Steegborn C, Schmidtmann A, Jaquet K, Pope B, Weeds AG.
Journal of Molecular Biology 2007 Feb; 366(3):745.
Application：WB, Recombinant protein.
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M.
Cell 2006 Nov; 127(3):635.
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Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K.
Nature 1998 Jun; 393(6687):809.
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Mapping the ADF/cofilin binding site on monomeric actin by competitive cross-linking and peptide array: evidence for a second binding site on monomeric actin.
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