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Last updated: 2016/12/4
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NCK2 monoclonal antibody, clone AF6D7

  • Catalog # : MAB2726
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant NCK2.
  • Immunogen:
  • Recombinant His fusion protein corresponding to human NCK2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody also react with NCK1.
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunoprecipitation (0.5 ul)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In HEPES, 150 mM NaCl (50% glycerol, 0.01% BSA, 0.03% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot analysis of cell lysates.
    Lane 1 : HeLa cell lysate.
    Lnae 2 : U-937 cell lysate.
    Lane 3 : A-431 cell lysate.
    Lane 4 : SK-N-MC cell lysate.
    Lane 5 : Ramos cell lysate.
    Lane 6 : PC-12 cell lysate.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 8440
  • Gene Name:
  • NCK2
  • Gene Alias:
  • GRB4,NCKbeta
  • Gene Description:
  • NCK adaptor protein 2
  • Gene Summary:
  • This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • SH2/SH3 adaptor protein NCK-beta,noncatalytic region of tyrosine kinase, beta
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