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Last updated: 2016/11/27
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GAPDH monoclonal antibody, clone 6C5

  • Catalog # : MAB2549
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native GAPDH.
  • Immunogen:
  • Native purified rabbit muscle GAPDH (whole molecule).
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 36
  • Reactivity:
  • Dog, Human, Mouse, Rabbit, Rat
  • Specificity:
  • This antibody recognizes GAPDH monomer (36 KDa) and the dimer form.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Antibody Reactive Against Native Purified Protein.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Tissue lysate from mouse muscle was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 500.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human colon tissue stained with GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
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