GAPDH monoclonal antibody, clone 6C5
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More Files
- More Functions
Western Blot (Tissue lysate)
Tissue lysate from mouse muscle was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon tissue stained with GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against native GAPDH.
Immunogen
Native purified rabbit muscle GAPDH (whole molecule).
Host
Mouse
Theoretical MW (kDa)
36
Reactivity
Dog, Human, Mouse, Rabbit, Rat
Specificity
This antibody recognizes GAPDH monomer (36 KDa) and the dimer form.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Flow Cytometry
Immunohistochemistry
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (Antibody Stabilizer).
Storage Instruction
Store at 4°C for one month. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Tissue lysate from mouse muscle was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 500.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon tissue stained with GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Publication Reference
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Loss of enhancer of zeste homologue 2 (EZH2) at tumor invasion front is correlated with higher aggressiveness in colorectal cancer cells.
Böhm J, Muenzner JK, Caliskan A, Ndreshkjana B, Erlenbach-Wünsch K, Merkel S, Croner R, Rau TT, Geppert CI, Hartmann A, Roehe AV, Schneider-Stock R.
Journal of Cancer Research and Clinical Oncology 2019 Sep; 145(9):2227.
Application：WB, Human, HCT-116 cells.
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Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping.
Munteanu B, von Reitzenstein C, Hänsch GM, Meyer B, Hopf C.
Analytical and Bioanalytical Chemistry 2012 Nov; 404(8):2277.
Application：WB-Ce, Human, HL-60 cells.
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Loss of enhancer of zeste homologue 2 (EZH2) at tumor invasion front is correlated with higher aggressiveness in colorectal cancer cells.
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