Mouse monoclonal antibody raised against native S100A1.
Native purified bovine brain S100A1.
Theoretical MW (kDa):
Bovine, Human, Mouse, Rat
This antibody recognizes 22 KDa for non-reduced S100A1 and 11 KDa for reduced S100A1.
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:50-1:100) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (BSA, 10% Proclin300)
Store at 4°C. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
Immunohistochemical staining of human melanoma with S100A1 monoclonal antibody, clone SPM354 (Cat # MAB2546) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.