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Last updated: 2016/9/25
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S100A1 monoclonal antibody, clone SPM354

  • Catalog # : MAB2546
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native S100A1.
  • Immunogen:
  • Native purified bovine brain S100A1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 22, 11
  • Reactivity:
  • Bovine, Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes 22 KDa for non-reduced S100A1 and 11 KDa for reduced S100A1.
  • Form:
  • Liquid
  • Isotype:
  • IgG2a, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Native Purified Protein.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:50-1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human melanoma with S100A1 monoclonal antibody, clone SPM354 (Cat # MAB2546) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • S100A1
  • Gene Alias:
  • MGC159706
  • Gene Description:
  • S100 calcium binding protein A1
  • Other Designations:
  • -
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