VEGFA monoclonal antibody, clone SPM225
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin-embedded human angiosarcoma stained with VEGFA monoclonal antibody, clone SPM225 (Cat # MAB2544) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant VEGFA.
Immunogen
Recombinant protein corresponding to human VEGFA.
Host
Mouse
Theoretical MW (kDa)
19-22
Reactivity
Human
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C to -80°C. Upon initial thawing, apportion into working aliquots and store at -80°C fot up to six months. The antibosies can be stored at 4°C for one month without detectable loss of activity.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin-embedded human angiosarcoma stained with VEGFA monoclonal antibody, clone SPM225 (Cat # MAB2544) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — VEGFA
Entrez GeneID
7422Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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Pathway
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Disease
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