IRAK4 (phospho T345) monoclonal antibody, clone A8A8
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More Files
- More Functions
Western Blot (Cell lysate)
The 5 x 107 Jurkat cells were stimulated by 50 ng/mL IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with Phosphospecific peptide. Lane 3 was pre-incubate with non-phospho specific peptide.An immunoreactive band is observed around ~51 KDa.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : IRAK4 (pT345). B : IRAK4 (non phosphorylated). Followed by IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) incubation at a 1 : 1000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of IRAK4.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T345 of human IRAK4.
Sequence
VMTSR
Host
Mouse
Reactivity
Bovine, Dog, Human, Mouse
Specificity
This antibody recognizes IRAK4 (phospho T345) with a phosphorylated site at Threonine 345. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The 5 x 107 Jurkat cells were stimulated by 50 ng/mL IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with Phosphospecific peptide. Lane 3 was pre-incubate with non-phospho specific peptide.An immunoreactive band is observed around ~51 KDa.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : IRAK4 (pT345). B : IRAK4 (non phosphorylated). Followed by IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) incubation at a 1 : 1000 dilution. -
Gene Info — IRAK4
Entrez GeneID
51135Gene Name
IRAK4
Gene Alias
IPD1, NY-REN-64, REN64
Gene Description
interleukin-1 receptor-associated kinase 4
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
Other Designations
IRAK-4 mutated form 1|interleukin-1 receptor associated kinase 4
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Interactome
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Pathway
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Disease
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Publication Reference
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IRAK4 mediates colitis-induced tumorigenesis and chemoresistance in colon cancer.
Li Q, Chen Y, Zhang D, Grossman J, Li L, Khurana N, Jiang H, Grierson P, Herndon J, DeNardo DG, Challen GA, Liu J, Ruzinova MB, Fields RC, Lim KH.
JCI Insight 2019 Oct; 4(19):130867.
Application：PLA, Human, KM-12, HCT-116, DLD-1 cells.
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Tumor-Stroma IL1β-IRAK4 Feedforward Circuitry Drives Tumor Fibrosis, Chemoresistance, and Poor Prognosis in Pancreatic Cancer.
Zhang D, Li L, Jiang H, Li Q, Wang-Gillam A, Yu J, Head R, Liu J, Ruzinova MB, Lim KH.
Cancer Research 2018 Apr; 78(7):1700.
Application：IF, IHC, Human, Pancreas, Pancreatitis, Pancreatic ductal adenocarcinoma, Capan-1 xenograft, KPC tumors.
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TLR signalling affects sperm mitochondrial function and motility via phosphatidylinositol 3-kinase and glycogen synthase kinase-3α.
Zhu X, Shi D, Li X, Gong W, Wu F, Xuejiang G, Xiao H, Liu L, Zhou H.
Cellular Signalling 2015 Mar; 28(3):148.
Application：WB, Mouse, Sperm.
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Augmentation of therapeutic responses in melanoma by inhibition of IRAK-1,-4.
Srivastava R, Geng D, Liu Y, Zheng L, Li Z, Joseph MA, McKenna C, Bansal N, Ochoa A, Davila E.
Cancer Research 2012 Dec; 72(23):6209.
Application：IHC-P,WB-Ce, Human, Malme-3M, SK-MEL-2, WM115, C32, RPMI-7951, A375, G361 cells, Melanoma.
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An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration.
Lehmann SM, Krüger C, Park B, Derkow K, Rosenberger K, Baumgart J, Trimbuch T, Eom G, Hinz M, Kaul D, Habbel P, Kälin R, Franzoni E, Rybak A, Nguyen D, Veh R, Ninnemann O, Peters O, Nitsch R, Heppner FL, Golenbock D, Schott E, Ploegh HL, Wulczyn FG, Lehnardt S.
Nature Neuroscience 2012 Jun; 15(6):827.
Application：IHC, Mouse, Mouse cortical neurons.
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IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase.
Li S, Strelow A, Fontana EJ, Wesche H.
PNAS 2002 Apr; 99(8):5567.
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IRAK4 mediates colitis-induced tumorigenesis and chemoresistance in colon cancer.
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