Mouse monoclonal antibody raised against native SPTA1.
Native purified human SPTA1.
This antibody is specific to the 240kD erythroid alpha-spectrin. Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific to erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics. Monoclonal antibody to erythroid alpha-spectrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (1% BSA, 0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq