SPTA1 monoclonal antibody, clone AF10
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Specification
Product Description
Mouse monoclonal antibody raised against native SPTA1.
Immunogen
Native purified human SPTA1.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to the 240kD erythroid alpha-spectrin. Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific to erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics. Monoclonal antibody to erythroid alpha-spectrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (1% BSA, 0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunoprecipitation
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Gene Info — SPTA1
Entrez GeneID
6708Gene Name
SPTA1
Gene Alias
EL2, SPTA
Gene Description
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
Gene Ontology
HyperlinkGene Summary
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq
Other Designations
OTTHUMP00000021115|alpha-I spectrin|erythrocyte alpha-spectrin|erythroid alpha-spectrin|spectrin alpha chain, erythrocyte|spectrin, alpha, erythrocytic 1
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Interactome
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Disease
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Publication Reference
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Expression of megakaryocytic and erythroid properties in human leukemic cells.
Tani T, Ylänne J, Virtanen I.
Exp Hematol 1996 Feb; 24(2):158.
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Expression of megakaryocytic and erythroid properties in human leukemic cells.
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