TNPO1 monoclonal antibody, clone D45
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant TNPO1.
Immunogen
Recombinant His fusion protein corresponding to human TNPO1.
Host
Mouse
Theoretical MW (kDa)
90
Reactivity
Human, Monkey, Rabbit
Specificity
Detects a band of approximately 90 KDa.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot for TNPO1 monoclonal antibody, clone D45 (Cat # MAB2496) on HeLa cell extracts.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TNPO1
Entrez GeneID
3842Gene Name
TNPO1
Gene Alias
IPO2, KPNB2, MIP, MIP1, TRN
Gene Description
transportin 1
Omim ID
602901Gene Ontology
HyperlinkGene Summary
This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq
Other Designations
M9 region interaction protein|OTTHUMP00000126960|importin 2|importin beta 2|karyopherin (importin) beta 2
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Interactome
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Publication Reference
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Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
Siomi MC, Eder PS, Kataoka N, Wan L, Liu Q, Dreyfuss G.
The Journal of Cell Biology 1997 Sep; 138(6):1181.
Application:WB-Ce, Human, Monkey, Quail, Xenopus, Rabbit, HeLa, COS, QT-6, XL177 cells, Reticulocytes.
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Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
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