HNRNPA1 monoclonal antibody, clone 9H10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against native HNRNPA1.
Immunogen
Native purified human HNRNPA1.
Host
Mouse
Theoretical MW (kDa)
34
Reactivity
Human
Specificity
Detects a band of approximately 34 KDa.
Form
Liquid
Isotype
IgG2b
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (1:2000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of HNRNPA1 monoclonal antibody, clone 9H10 (Cat # MAB2492) on HeLa cell extract.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HNRNPA1
Entrez GeneID
3178Gene Name
HNRNPA1
Gene Alias
HNRPA1, MGC102835
Gene Description
heterogeneous nuclear ribonucleoprotein A1
Omim ID
164017Gene Ontology
HyperlinkGene Summary
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq
Other Designations
helix-destabilizing protein|heterogeneous nuclear ribonucleoprotein A1B protein|heterogeneous nuclear ribonucleoprotein B2 protein|heterogeneous nuclear ribonucleoprotein core protein A1|nuclear ribonucleoprotein particle A1 protein|single-strand DNA-bind
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Interactome
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Disease
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Publication Reference
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P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.
Bugai A, Quaresma AJC, Friedel CC, Lenasi T, Düster R, Sibley CR, Fujinaga K, Kukanja P, Hennig T, Blasius M, Geyer M, Ule J, Dölken L, Barborič M.
Molecular Cell 2019 Apr; 74(2):254.
Application:WB-Tr, Human, HEK 293 cells.
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HnRNPH1/H2, U1 snRNP, and U11 snRNP cooperate to regulate the stability of the U11-48K pre-mRNA.
Turunen JJ, Verma B, Nyman TA, Frilander MJ.
RNA 2013 Mar; 19(3):380.
Application:WB-IP, Human, Hela cells.
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hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing.
Kashima T, Rao N, David CJ, Manley JL.
Human Molecular Genetics 2007 Dec; 16(24):3149.
Application:IP, WB-Tr, Human, HeLa cells.
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RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
Burd CG, Dreyfuss G.
The EMBO Journal 1994 Mar; 13(5):1197.
Application:IP, WB-Ce, Human, HeLa cells, Recombinant proteins.
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P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.
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