NEDD9 monoclonal antibody, clone 2G9
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NEDD9.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 82-398 of human NEDD9.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
human, mouse, rat NEDD9. Does not recognize p130Cas. Have not tested on Sin1. This mAb mostly detects HEF1 localized at the focal adhesion sites.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
Immunoprecipitation (1:2000-1:6000)
Immunofluorescence (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — NEDD9
Entrez GeneID
4739Gene Name
NEDD9
Gene Alias
CAS-L, CAS2, CASL, CASS2, HEF1, dJ49G10.2, dJ761I2.1
Gene Description
neural precursor cell expressed, developmentally down-regulated 9
Omim ID
602265Gene Ontology
HyperlinkGene Summary
O
Other Designations
Cas scaffolding protein family member 2|Crk-associated substrate related|OTTHUMP00000016030|cas-like docking|dJ49G10.2 (Enhancer of Filamentation 1 (HEF1))|dJ761I2.1 (enhancer of filamentation (HEF1))|enhancer of filamentation 1
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Interactome
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Disease
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Publication Reference
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NEDD9 overexpression correlates with poor prognosis in gastric cancer.
Liu Y, Wang D, Zhao KL, Zhu JW, Yin HB, Wei YZ, Wu ZJ, Cheng GJ, Wang F, Ni F, Wang XL, Cao GX, Huang J, Cai J.
Tumour Biology 2014 Jul; 35(7):6351.
Application:IHC, Human, Gastric.
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The hematopoietic isoform of Cas-Hef1-associated signal transducer regulates chemokine-induced inside-out signaling and T cell trafficking.
Regelmann AG, Danzl NM, Wanjalla C, Alexandropoulos K.
Immunity 2006 Dec; 25(6):907.
Application:IP, Mouse, Mouse T cells.
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The Cas family docking protein, HEF1, promotes the formation of neurite-like membrane extensions.
Bargon SD, Gunning PW, O'Neill GM.
Biochimica et Biophysica Acta 2005 Dec; 1746(2):143.
Application:IS, WB-Tr, Human, HEF1.M1, HEF1.M2 cells.
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Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics.
O'Neill GM, Golemis EA.
Mol Cell Biol 2001 Aug; 21(15):5094.
Application:WB, Human, MCF-7 cells.
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NEDD9 overexpression correlates with poor prognosis in gastric cancer.
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