Epithelial keratins monoclonal antibody, clone AE5
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Specification
Product Description
Mouse monoclonal antibody raised against native rabbit corneal epithelial keratins.
Immunogen
Native purified rabbit corneal epithelial keratins.
Host
Mouse
Reactivity
Bovine, Human, Rabbit
Specificity
The 64K basic keratin K3.
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Publication Reference
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Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.
Raeder S, Utheim TP, Utheim OA, Nicolaissen B, Roald B, Cai Y, Haug K, Kvalheim A, Messelt EB, Drolsum L, Reed JC, Lyberg T.
Investigative Ophthalmology & Visual Science 2007 Dec; 48(12):5484.
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Effect of limbal explant orientation on the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells.
Raeder S, Utheim TP, Utheim OA, Cai Y, Roald B, Lyberg T, Kjeldsen-Kragh J, Ramstad H, Messelt E, Nicolaissen B Jr.
Acta Ophthalmologica Scandinavica 2007 Jun; 85(4):377.
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Regulation of K3 keratin gene transcription by Sp1 and AP-2 in differentiating rabbit corneal epithelial cells.
Chen TT, Wu RL, Castro-Munozledo F, Sun TT.
Molecular and Cellular Biology 1997 Jun; 17(6):3056.
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Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.
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