Cdkn2a monoclonal antibody, clone 12-A1-1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2agene (lane 1) and from wild type MEF.
Immunofluorescence
Immunofluorescent detection of Cdkn2a in wild type MEF at passage 5 (images 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (images 3, 4) using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416).
Immunoprecipitation
Immunoprecipitation of Cdkn2a monoclonal antibody, clone 12-A1-1 using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a.
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Specification
Product Description
Rat monoclonal antibody raised against synthetic peptide of Cdkn2a.
Immunogen
A synthetic peptide corresponding to amino acids 1-100 of mouse Cdkn2a.
Host
Rat
Reactivity
Mouse
Specificity
This antibody is specific to the epitope residues 54-62 of murine p19ARF. This region is encoded by 1 beta of the mouse Arf gene. It does not recognize human p14Arf nor Golden hamster p13Arf.
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:500)
Immunofluorescence (1:500)
Immunohistochemistry (Frozen sections) (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500)
Immunoprecipitation (1:500)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2agene (lane 1) and from wild type MEF.Immunofluorescence
Immunofluorescent detection of Cdkn2a in wild type MEF at passage 5 (images 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (images 3, 4) using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416).Immunoprecipitation
Immunoprecipitation of Cdkn2a monoclonal antibody, clone 12-A1-1 using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a. -
Gene Info — Cdkn2a
Entrez GeneID
12578Protein Accession#
Q64364Gene Name
Cdkn2a
Gene Alias
ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF
Gene Description
cyclin-dependent kinase inhibitor 2A
Gene Ontology
HyperlinkGene Summary
inhibits CDK4)|cyclin-dependent kinase inhibitor protein|mitochondrial smARF
Other Designations
OTTMUSP00000008143|cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)|cyclin-dependent kinase inhibitor protein|mitochondrial smARF
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Publication Reference
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A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
Apicelli AJ, Maggi LB Jr, Hirbe AC, Miceli AP, Olanich ME, Schulte-Winkeler CL, Saporita AJ, Kuchenreuther M, Sanchez J, Weilbaecher K, Weber JD.
Molecular and Cellular Biology 2007 Dec; 28(3):1068.
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Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Klochendler-Yeivin A, Picarsky E, Yaniv M.
Molecular and Cellular Biology 2006 Apr; 26(7):2661.
Application：WB-Ce, Mouse, MEFs.
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Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF.
Hybridoma and Hybridomics 2004 Oct; 23(5):293.
Application：IF, IP, IHC, WB, Mouse, NIH/3T3 cells.
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A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
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