Rat monoclonal antibody raised against synthetic peptide of Cdkn2a.
A synthetic peptide corresponding to amino acids 1-100 of mouse Cdkn2a.
This antibody is specific to the epitope residues 54-62 of murine p19ARF. This region is encoded by 1 beta of the mouse Arf gene. It does not recognize human p14Arf nor Golden hamster p13Arf.
ELISA (1:500) Immunofluorescence (1:500) Immunohistochemistry (Frozen sections) (1:500) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500) Immunoprecipitation (1:500) Western Blot (1:500) The optimal working dilution should be determined by the end user.
In buffer containing 0.09% sodium azide
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2agene (lane 1) and from wild type MEF.
Immunofluorescent detection of Cdkn2a in wild type MEF at passage 5 (images 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (images 3, 4) using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416).
Immunoprecipitation of Cdkn2a monoclonal antibody, clone 12-A1-1 using Cdkn2a monoclonal antibody, clone 12-A1-1 (Cat # MAB2416). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a.