Western blot analysis of RPS27A using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414). Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3, 4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively. 20% gels with PVDF blots (Nitrocellulose works but not as well).