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RPS27A monoclonal antibody, clone Ubi-1

  • Catalog # : MAB2414
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against RPS27A.
  • Immunogen:
  • Purified ubiquitin conjugated with KLH with glutaraldehyde.
  • Host:
  • Mouse
  • Reactivity:
  • Bovine, Chicken, Fruit fly, Human, Mouse, Nematoda, Plants
  • Specificity:
  • This antibody specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against UBC.
  • Recommend Usage:
  • Immunofluorescence (1:500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In buffer containing 5 mM sodium azide
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of RPS27A using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414). Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3, 4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively. 20% gels with PVDF blots (Nitrocellulose works but not as well).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of RPS27A in hippocampal tissue from an Alzheimer patient, using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414).
  • Immunofluorescence
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • ELISA
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