rRNA monoclonal antibody, clone Y10b
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Specification
Product Description
Mouse monoclonal antibody raised against native rRNA.
Immunogen
Native purified 5.8S ribosomal RNA.
Host
Mouse
Specificity
This antibody is specific to ribosomal RNA. There is no cross-reactivity with other RNAs.
Form
Liquid
Isotype
IgG3, kappa
Quality Control Testing
Antibody Reactive Against rRNA.
Recommend Usage
Immunoprecipitation (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1 M Tris, 1.5 M NaCl, 100 mM glycine (0.05% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescent imaging of rRNA in vero cells using rRNA monoclonal antibody (Cat # MAB2412).Immunoprecipitation
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Publication Reference
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Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes.
Thomas MG, Martinez Tosar LJ, Loschi M, Pasquini JM, Correale J, Kindler S, Boccaccio GL.
Molecular Biology of the Cell 2005 Jan; 16(1):405.
Application:IF, Rat, Oligodendrocytes.
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Axonal defects in mouse models of motoneuron disease.
Jablonka S, Wiese S, Sendtner M.
Journal of Neurobiology 2004 Feb; 58(2):272.
Application:IF, Mouse, Embryonic motoneurons.
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Increased expression and localization of the RNA-binding protein HuD and GAP-43 mRNA to cytoplasmic granules in DRG neurons during nerve regeneration.
Anderson KD, Merhege MA, Morin M, Bolognani F, Perrone-Bizzozero NI.
Experimental Neurology 2003 Sep; 183(1):100.
Application:IF, Rat, Rat DRG neurons.
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Ribosomes in the squid giant axon.
Bleher R, Martin R.
Neuroscience 2001 Nov; 107(3):527.
Application:IF, Loligo, Giant axon.
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Dynamic visualization of local protein synthesis in hippocampal neurons.
Aakalu G, Smith WB, Nguyen N, Jiang C, Schuman EM.
Neuron 2001 May; 30(2):489.
Application:IF, Rat, Rat hippocampal neuron.
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Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes.
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