Western blot analysis of CAV1 in NIH/3T3 cell lysates (50 ug) using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408). Lanes 1 and 2 : 1 : 4,000. Lanes 3 and 4 : 1 : 1,000. ECL : 5 minute exposure.
Immunofluorescent imaging using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408) at 1 : 200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane (Photo courtesy of Alberto Davalos, Yale School of Medicine).
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq