3-OHKYN monoclonal antibody, clone P3UI
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Specification
Product Description
Mouse monoclonal antibody raised against 3-OHKYN.
Immunogen
3-OHKYN modified KLH.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to 3-hydroxykynureinine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against 3-OHKYN.
Recommend Usage
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot analysis of 3-OHKYN in modified BSA using 3-OHKYN monoclonal antibody, clone P3UI (Cat # MAB2404). 10 second ECL exposure. Lane 1 : BSA (-), Lane 2 : BSA (+).Immunocytochemistry
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Publication Reference
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3-hydroxykynurenine-mediated modification of human lens proteins: structure determination of a major modification using a monoclonal antibody.
Staniszewska MM, Nagaraj RH.
The Journal of Biological Chemistry 2005 Apr; 280(23):22154.
Application:ELISA, IF, IHC-P, WB, Human, HLE-B3 cells, Human lenses.
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3-hydroxykynurenine-mediated modification of human lens proteins: structure determination of a major modification using a monoclonal antibody.
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