BRIP1 monoclonal antibody, clone pp15-IB4
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More Files
- More Functions
Western Blot (Cell lysate)
BRIP1 detected in 293 cell lysate using BRIP1 monoclonal antibody, clone pp15-IB4 (Cat # MAB2402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer using 1:200 dilution of BRIP1 monoclonal antibody, clone pp15-IB4 (Cat # MAB2402). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of BRIP1.
Immunogen
A synthetic peptide corresponding to residues within amino acids 1200-1249 at C-terminus of human BRIP1.
Host
Mouse
Theoretical MW (kDa)
130
Reactivity
Human
Specificity
This antibody is specific to Brip1/FANCJ.
Form
Liquid
Purity
Unpurified.
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation (1:10-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.05% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
BRIP1 detected in 293 cell lysate using BRIP1 monoclonal antibody, clone pp15-IB4 (Cat # MAB2402).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer using 1:200 dilution of BRIP1 monoclonal antibody, clone pp15-IB4 (Cat # MAB2402). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.Immunoprecipitation
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Gene Info — BRIP1
Entrez GeneID
83990Protein Accession#
Q9BX63Gene Name
BRIP1
Gene Alias
BACH1, FANCJ, FLJ90232, MGC126521, MGC126523, OF
Gene Description
BRCA1 interacting protein C-terminal helicase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq
Other Designations
BRCA1-associated C-terminal helicase 1|BRCA1-binding helicase-like protein BACH1|BRCA1-interacting protein 1
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Interactome
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Disease
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Publication Reference
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Cell cycle-dependent chromatin loading of the Fanconi anemia core complex by FANCM/FAAP24.
Kim JM, Kee Y, Gurtan A, D'Andrea AD.
Blood 2008 May; 111(10):5215.
Application：WB, Human, HeLa cells.
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Characterization of the interactome of the human MutL homologues MLH1, PMS1, and PMS2.
Cannavo E, Gerrits B, Marra G, Schlapbach R, Jiricny J.
The Journal of Biological Chemistry 2007 Feb; 282(5):2976.
Application：IP, WB, Human, HeLa cells.
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BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function.
Cantor SB, Bell DW, Ganesan S, Kass EM, Drapkin R, Grossman S, Wahrer DC, Sgroi DC, Lane WS, Haber DA, Livingston DM.
Cell 2001 Apr; 105(1):149.
Application：IF, WB-Tr, Human, HCC1937, MCF-7 cells.
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Cell cycle-dependent chromatin loading of the Fanconi anemia core complex by FANCM/FAAP24.
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