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BRIP1 monoclonal antibody, clone pp15-IB4 

  • Catalog # : MAB2402
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of BRIP1.
  • Immunogen:
  • A synthetic peptide corresponding to residues within amino acids 1200-1249 at C-terminus of human BRIP1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 130
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to Brip1/FANCJ.
  • Form:
  • Liquid
  • Purity:
  • Unpurified.
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunoprecipitation (1:10-1:500)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • BRIP1 detected in 293 cell lysate using BRIP1 monoclonal antibody, clone pp15-IB4 (Cat # MAB2402).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer using 1:200 dilution of BRIP1 monoclonal antibody, clone pp15-IB4 (Cat # MAB2402). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • BRIP1
  • Gene Alias:
  • BACH1,FANCJ,FLJ90232,MGC126521,MGC126523,OF
  • Gene Description:
  • BRCA1 interacting protein C-terminal helicase 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq
  • Other Designations:
  • BRCA1-associated C-terminal helicase 1,BRCA1-binding helicase-like protein BACH1,BRCA1-interacting protein 1
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