RPE65 monoclonal antibody, clone 401.8B11.3D9
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More Files
- More Functions
Western Blot
RPE65 detected in bovine and human samples using RPE65 monoclonal antibody, clone 401.8B11.3D9 (Cat # MAB2397). Lane 1 : bovine RPE membrane, lane 2 : recombinant human RPE transfected COS-7 cell lysate.
Immunohistochemistry
Immunohistochemical staining of RPE65 in mouse retina tissue using RPE65 monoclonal antibody, clone 401.8B11.3D9 (Cat # MAB2397).
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Specification
Product Description
Mouse monoclonal antibody raised against native RPE65.
Immunogen
Native purified from bovine RPE microsomal membrane proteins.
Host
Mouse
Reactivity
Bovine, Human, Mouse, Pig
Specificity
This antibody is specific to RPE65.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (Frozen sections) (1:250)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
RPE65 detected in bovine and human samples using RPE65 monoclonal antibody, clone 401.8B11.3D9 (Cat # MAB2397). Lane 1 : bovine RPE membrane, lane 2 : recombinant human RPE transfected COS-7 cell lysate.Immunohistochemistry (Frozen sections)
Immunohistochemistry
Immunohistochemical staining of RPE65 in mouse retina tissue using RPE65 monoclonal antibody, clone 401.8B11.3D9 (Cat # MAB2397).Immunocytochemistry
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Gene Info — RPE65
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Publication Reference
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A survey of vitreous cell components performed using liquid-based cytology.
Narumi M, Nishitsuka K, Yamakawa M, Yamashita H.
Acta Ophthalmologica 2015 Aug; 93(5):e386.
Application：ICC, Human, Retinal pigment epithelium cells.
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CD133 progenitor cells from the bone marrow contribute to retinal pigment epithelium repair.
Harris JR, Fisher R, Jorgensen M, Kaushal S, Scott EW.
Stem Cells 2009 Feb; 27(2):457.
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Characterization of a spontaneous mouse retinal pigment epithelial cell line B6-RPE07.
Chen M, Muckersie E, Robertson M, Fraczek M, Forrester JV, Xu H.
Investigative Ophthalmology & Visual Science 2008 Aug; 49(8):3699.
Application：WB-Ce, Mouse, B6-RPE07 cells.
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Reactivation of uveitogenic T cells by retinal astrocytes derived from experimental autoimmune uveitis-prone B10RIII mice.
Jiang G, Ke Y, Sun D, Han G, Kaplan HJ, Shao H.
Investigative Ophthalmology & Visual Science 2008 Jan; 49(1):282.
Application：IF, Mouse, Mouse retinal pigment epithelial (RPE) cells.
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Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase.
Lall MM, Ferrell J, Nagar S, Fleisher LN, McGahan MC.
Investigative Ophthalmology & Visual Science 2008 Jan; 49(1):310.
Application：IF, IHC-P, Mouse, Mouse retina, retinal pigment epithelial (RPE) cells.
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A survey of vitreous cell components performed using liquid-based cytology.
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