EP300 monoclonal antibody, clone RW128
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of EP300 in a HeLa nuclear extract using EP300 monoclonal antibody, clone RW128 (Cat # MAB2384) (1 : 250). ECL : 15 second exposure.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EP300.
Immunogen
Recombinant protein corresponding to amino acids 1572-2371 of human EP300.
Host
Mouse
Reactivity
Human, Mouse, Primates, Rat
Specificity
This antibody is specific to p300 protein. This antibody recognizes residues 1868-1921.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EP300 in a HeLa nuclear extract using EP300 monoclonal antibody, clone RW128 (Cat # MAB2384) (1 : 250). ECL : 15 second exposure.Immunofluorescence
Immunoprecipitation
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Gene Info — EP300
Entrez GeneID
2033Gene Name
EP300
Gene Alias
KAT3B, p300
Gene Description
E1A binding protein p300
Gene Ontology
HyperlinkGene Summary
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq
Other Designations
E1A-associated protein p300|E1A-binding protein, 300kD|OTTHUMP00000028668|histone acetyltransferase p300
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Interactome
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Pathway
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Disease
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Publication Reference
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m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3.
Ting Shan, Feiyan Liu, Miaomiao Wen, Zonggui Chen, Shaopeng Li, Yafen Wang, Hong Cheng, Yu Zhou.
Molecular Cell 2023 Dec; 83(24):4494.
Application：WB, Human, HeLa cells.
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Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
Eckner R, Yao TP, Oldread E, Livingston DM.
Genes & Development 1996 Oct; 10(19):2478.
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Association of p300 and CBP with simian virus 40 large T antigen.
Eckner R, Ludlow JW, Lill NL, Oldread E, Arany Z, Modjtahedi N, DeCaprio JA, Livingston DM, Morgan JA.
Molecular and Cellular Biology 1996 Jul; 16(7):3454.
Application：IP, Human, Mouse, MEF, 293, U2OS cells.
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Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM.
Genes & Development 1994 Apr; 8(8):869.
Application：IP, Human, U-2 OS cells.
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m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3.
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