UCHL1 monoclonal antibody, clone BH7
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blots of UCHL1 in whole cell homogenate of bovine brain stained with UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (right most lane), and two other monoclonal antibodies reactive with UCHL1 (left and central lane).
Immunofluorescence
Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (green) and rabbit antibody to neurofilament NF-M (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant UCHL1.
Immunogen
Recombinant protein corresponding to full length human UCHL1.
Host
Mouse
Reactivity
Bovine, Human, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:1000)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (10 mM sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blots of UCHL1 in whole cell homogenate of bovine brain stained with UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (right most lane), and two other monoclonal antibodies reactive with UCHL1 (left and central lane).Immunofluorescence
Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (green) and rabbit antibody to neurofilament NF-M (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments. -
Gene Info — UCHL1
Entrez GeneID
7345Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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Interactome
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Disease
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Publication Reference
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The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr.
Cell 2002 Oct; 111(2):209.
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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL.
FASEB Journal 2002 Apr; 16(8):869.
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The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.
K D Wilkinson, K M Lee, S Deshpande, P Duerksen-Hughes, J M Boss, J Pohl.
Science 1989 Nov; 46(4930):670.
Application：AFC, WB-Re, WB-Ti, Bovine, Bovine thymus, Recombinant proteins.
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The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
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