Western blots of UCHL1 in whole cell homogenate of bovine brain stained with UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (right most lane), and two other monoclonal antibodies reactive with UCHL1 (left and central lane).
Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (green) and rabbit antibody to neurofilament NF-M (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease