TERT monoclonal antibody, clone 2D8
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More Files
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Western Blot (Cell lysate)
Western blot analysis of human TERT using TERT monoclonal antibody, clone 2D8 (Cat # MAB2379). Sample : MJ90 whole cell lysate.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant TERT.
Immunogen
Recombinant protein corresponding to full length human TERT.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to hTERT.
Form
Liquid
Isotype
IgM
Recommend Usage
Flow Cytometry (1:50-1:200)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human TERT using TERT monoclonal antibody, clone 2D8 (Cat # MAB2379). Sample : MJ90 whole cell lysate.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — TERT
Entrez GeneID
7015Gene Name
TERT
Gene Alias
EST2, TCS1, TP2, TRT, hEST2
Gene Description
telomerase reverse transcriptase
Gene Ontology
HyperlinkGene Summary
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq
Other Designations
telomerase catalytic subunit
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Interactome
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Disease
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Publication Reference
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Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.
Zhang JG, Eguchi J, Kruse CA, Gomez GG, Fakhrai H, Schroter S, Ma W, Hoa N, Minev B, Delgado C, Wepsic HT, Okada H, Jadus MR.
Clinical Cancer Research 2007 Jan; 13(2 Pt 1):566.
Application：Flow Cyt, Human, U-251 cells.
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The telomerase reverse transcriptase regulates chromatin state and DNA damage responses.
Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC.
PNAS 2005 Jun; 102(23):8222.
Application：WB, Human, BJ fibroblasts.
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Telomerase maintains telomere structure in normal human cells.
Masutomi K, Yu EY, Khurts S, Ben-Porath I, Currier JL, Metz GB, Brooks MW, Kaneko S, Murakami S, DeCaprio JA, Weinberg RA, Stewart SA, Hahn WC.
Cell 2003 Jul; 114(2):241.
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Evolving views of telomerase and cancer.
Blasco MA, Hahn WC.
Trends in Cell Biology 2003 Jun; 13(6):289.
Application：WB, Human, Cancers, Mammalian cells.
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Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.
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