ARNT monoclonal antibody, clone H1beta234
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ARNT.
Immunogen
Recombinant protein corresponding to amino acids 496-789 of human ARNT.
Host
Mouse
Reactivity
Bovine, Domestic ferret, Human, Mouse, Rat, Sheep
Specificity
This antibody is specific to HIF-1 beta /ARNT.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ARNT on human glioblastoma multi-forme using ARNT monoclonal antibody, clone H1beta234 (Cat # MAB2370). -
Gene Info — ARNT
Entrez GeneID
405Protein Accession#
P27540Gene Name
ARNT
Gene Alias
HIF-1beta, HIF1B, HIF1BETA, TANGO, bHLHe2
Gene Description
aryl hydrocarbon receptor nuclear translocator
Omim ID
126110Gene Ontology
HyperlinkGene Summary
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000032943|dioxin receptor, nuclear translocator|hypoxia-inducible factor 1, beta subunit
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Interactome
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Pathway
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Publication Reference
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TLR2 controls intestinal carcinogen detoxication by CYP1A1.
Do KN, Fink LN, Jensen TE, Gautier L, Parlesak A.
PLoS One 2012 Mar; 7(3):e32309.
Application:WB-Ti, Mouse, Mouse liver, small intestines.
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Antioxidants, HIF prolyl hydroxylase inhibitors or short interfering RNAs to BNIP3 or PUMA, can prevent prodeath effects of the transcriptional activator, HIF-1alpha, in a mouse hippocampal neuronal line.
Aminova LR, Siddiq A, Ratan RR.
Antioxidants & Redox Signaling 2008 Dec; 10(12):1989.
Application:WB, Mouse, HT22 cells.
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Lung development in the Holtzman rat is adversely affected by gestational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Kransler KM, McGarrigle BP, Swartz DD, Olson JR.
Toxicological Sciences: an Official Journal of the Society of Toxicology 2009 Feb; 107(2):498.
Application:IHC-P, Rat, Rat lung.
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Topotecan inhibits vascular endothelial growth factor production and angiogenic activity induced by hypoxia in human neuroblastoma by targeting hypoxia-inducible factor-1alpha and -2alpha.
Puppo M, Battaglia F, Ottaviano C, Delfino S, Ribatti D, Varesio L, Bosco MC.
Molecular Cancer Therapeutics 2008 Jul; 7(7):1974.
Application:WB-Ce, WB-Tr, Human, GI-LI-N, LAN-5 cells.
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(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors.
Lauzier MC, Robitaille GA, Chan DA, Giaccia AJ, Richard DE.
Molecular Pharmacology 2008 Apr; 74(1):282.
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Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha.
Baek JH, Liu YV, McDonald KR, Wesley JB, Hubbi ME, Byun H, Semenza GL.
The Journal of Biological Chemistry 2007 Aug; 282(32):23572.
Application:WB-Tr, Human, HEK 293 cells.
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TLR2 controls intestinal carcinogen detoxication by CYP1A1.
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