CASP8 monoclonal antibody, clone FLICE 4-1-20
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant CASP8.
Immunogen
Recombinant protein corresponding to full length human CASP8.
Host
Mouse
Reactivity
Human
Specificity
Specific for Caspase 8.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot detection of CASP8 (59 kDa) from 293 cell extract using CASP8 monoclonal antibody, clone FLICE 4-1-20 (Cat #MAB2358) (1 : 500).
Lanes 1 and 2 contain inactive and active CASP8, respectively. -
Gene Info — CASP8
Entrez GeneID
841Protein Accession#
Q14790Gene Name
CASP8
Gene Alias
ALPS2B, CAP4, FLICE, FLJ17672, MACH, MCH5, MGC78473
Gene Description
caspase 8, apoptosis-related cysteine peptidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq
Other Designations
FADD-homologous ICE/CED-3-like protease|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|Mch5 isoform alpha|OTTHUMP00000163718|OTTHUMP00000163719|OTTHUMP00000163720|caspase 8|caspase 8, apoptosis-related cysteine protease|cysteine protease|procaspase-8
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Interactome
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Pathway
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Disease
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Publication Reference
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Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c.
Kirsch DG, Doseff A, Chau BN, Lim DS, de Souza-Pinto NC, Hansford R, Kastan MB, Lazebnik YA, Hardwick JM.
The Journal of Biological Chemistry 1999 Jul; 274(30):21155.
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Oncogene-dependent apoptosis is mediated by caspase-9.
Fearnhead HO, Rodriguez J, Govek EE, Guo W, Kobayashi R, Hannon G, Lazebnik YA.
PNAS 1998 Nov; 95(23):13664.
Application:WB-Ce, Human, IMR-90-E1A cells.
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Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c.
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