CHAF1A monoclonal antibody, clone SS 1 1-13
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of CHAF1A on HeLa cells using CHAF1A monoclonal antibody, clone SS 1 1-13 (Cat #MAB2356) (Shibahara et al 1999).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant CHAF1A.
Immunogen
Recombinant protein corresponding to human CHAF1A.
Host
Mouse
Reactivity
Human
Specificity
Specific for CAF-1p150.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of CHAF1A on HeLa cells using CHAF1A monoclonal antibody, clone SS 1 1-13 (Cat #MAB2356) (Shibahara et al 1999).Immunoprecipitation
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Gene Info — CHAF1A
Entrez GeneID
10036Protein Accession#
Q13111Gene Name
CHAF1A
Gene Alias
CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150
Gene Description
chromatin assembly factor 1, subunit A (p150)
Omim ID
601246Gene Ontology
HyperlinkGene Summary
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM
Other Designations
chromatin assembly factor I (150 kDa)
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Interactome
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Disease
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Publication Reference
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In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells.
Galvani A, Courbeyrette R, Agez M, Ochsenbein F, Mann C, Thuret JY.
Molecular and Cellular Biology 2008 Jun; 28(11):3672.
Application：WB-Ce, Human, HeLa cells.
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Hsp60 regulation of tumor cell apoptosis.
Ghosh JC, Dohi T, Kang BH, Altieri DC.
The Journal of Biological Chemistry 2008 Feb; 283(8):5188.
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Proliferating cell nuclear antigen (PCNA) may function as a double homotrimer complex in the mammalian cell.
Naryzhny SN, Zhao H, Lee H.
The Journal of Biological Chemistry 2005 Apr; 280(14):13888.
Application：IP, WB-Tr, Mouse, CHO cells.
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Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin.
Shibahara K, Stillman B.
Cell 1999 Feb; 96(4):575.
Application：IF, IP, WB-Ce, Human, HeLa cells.
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Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro.
Smith S, Stillman B.
The Journal of Biological Chemistry 1991 Jun; 266(18):12041.
Application：ICC, IP, WB-Ce, WB-Re, Human, HEK 293 cells, HeLa cells, Recombinant protein.
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In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells.
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