CHAF1B monoclonal antibody, clone SS 53
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of :
A. RKO cells with p150 antibody and CHAF1B monoclonal antibody, clone SS 53 (Cat #MAB2355).
B. p150 RNAi knockdown.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant CHAF1B.
Immunogen
Recombinant protein corresponding to human CHAF1B.
Host
Mouse
Reactivity
Human
Specificity
Specific for CAF-1p60.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of :
A. RKO cells with p150 antibody and CHAF1B monoclonal antibody, clone SS 53 (Cat #MAB2355).
B. p150 RNAi knockdown.Immunoprecipitation
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Gene Info — CHAF1B
Entrez GeneID
8208Protein Accession#
Q13112Gene Name
CHAF1B
Gene Alias
CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Gene Description
chromatin assembly factor 1, subunit B (p60)
Omim ID
601245Gene Ontology
HyperlinkGene Summary
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq
Other Designations
CAF-1 subunit B|CAF-I 60 kDa subunit|M-phase phosphoprotein 7|chromatin assembly factor 1 subunit B|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
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Interactome
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Publication Reference
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In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells.
Galvani A, Courbeyrette R, Agez M, Ochsenbein F, Mann C, Thuret JY.
Molecular and Cellular Biology 2008 Jun; 28(11):3672.
Application：WB-Ce, Human, HeLa cells.
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Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo.
Green CM, Almouzni G.
The EMBO Journal 2003 Oct; 22(19):5163.
Application：IF, Human, Fibroblasts, HeLa cells.
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Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin.
Shibahara K, Stillman B.
Cell 1999 Feb; 96(4):575.
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Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro.
Smith S, Stillman B.
The Journal of Biological Chemistry 1991 Jun; 266(18):12041.
Application：ICC, IP, WB-Ce, WB-Re, Human, HEK 293 cells, HeLa cells, Recombinant protein.
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In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells.
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