EPAS1 monoclonal antibody, clone ep190b
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant EPAS1.
Immunogen
Recombinant protein corresponding to amino acids 535-631 of human EPAS1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to HIF-2 alpha and does not cross-react with HIF-1 alpha.
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:1000)
Flow Cytometry (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:150-1:300)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — EPAS1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours.
Kubo T, Sugita T, Shimose S, Matsuo T, Arihiro K, Ochi M.
The Journal of Bone and Joint Surgery. British Volume 2008 Mar; 90(3):364.
Application:IHC-P, Human, Human chondrosarcoma.
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Cyr61/CCN1 and CTGF/CCN2 mediate the proangiogenic activity of VHL-mutant renal carcinoma cells.
Chintalapudi MR, Markiewicz M, Kose N, Dammai V, Champion KJ, Hoda RS, Trojanowska M, Hsu T.
Carcinogenesis 2008 Apr; 29(4):696.
Application:WB-Tr, Human, 786-0 cells.
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Targeting the loss of the von Hippel-Lindau tumor suppressor gene in renal cell carcinoma cells.
Sutphin PD, Chan DA, Li JM, Turcotte S, Krieg AJ, Giaccia AJ.
Cancer Research 2007 Jun; 67(12):5896.
Application:WB, Human, RCC4 cells.
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Involvement of hypoxia-inducible transcription factors in polycystic kidney disease.
Bernhardt WM, Wiesener MS, Weidemann A, Schmitt R, Weichert W, Lechler P, Campean V, Ong AC, Willam C, Gretz N, Eckardt KU.
The American Journal of Pathology 2007 Mar; 170(3):830.
Application:IHC-P, Human, Rat, Human, Rat kidney.
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Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha.
Wiesener MS, Turley H, Allen WE, Willam C, Eckardt KU, Talks KL, Wood SM, Gatter KC, Harris AL, Pugh CW, Ratcliffe PJ, Maxwell PH.
Blood 1998 Oct; 92(7):2260.
Application:WB-Ce, WB-Tr, Human, Monkey, COS-1, HT29, 293, Hep3B, HeLa, HMEC-1, MRC5 cells.
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Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours.
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