ESPL1 monoclonal antibody, clone XJ11-4D7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Overlay (blue) of centrosomal staining in HeLa cells. Centrosomal staining of ESPL1 (yellow), using ESPL1 monoclonal antibody, clone XJ11-4D7 (Cat # MAB2347), and gamma tubulin (green) in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ESPL1.
Immunogen
Recombinant fusion protein corresponding to amino acids 1866-1996 of human ESPL1.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to human separase.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Immunofluorescence
Overlay (blue) of centrosomal staining in HeLa cells. Centrosomal staining of ESPL1 (yellow), using ESPL1 monoclonal antibody, clone XJ11-4D7 (Cat # MAB2347), and gamma tubulin (green) in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells. -
Gene Info — ESPL1
Entrez GeneID
9700Protein Accession#
Q14674Gene Name
ESPL1
Gene Alias
ESP1, FLJ46492, KIAA0165, SEPARASE, SEPARIN
Gene Description
extra spindle pole bodies homolog 1 (S. cerevisiae)
Omim ID
604143Gene Ontology
HyperlinkGene Summary
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
Other Designations
extra spindle poles like 1|separin, separase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner.
Kim HS, Jeon YK, Ha GH, Park HY, Kim YJ, Shin HJ, Lee CG, Chung DH, Lee CW.
Cancer Research 2009 Jan; 69(1):27.
-
Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells.
Fujita T, Epperly MW, Zou H, Greenberger JS, Wan Y.
Molecular Biology of the Cell 2008 Dec; 19(12):5446.
Application：IP, WB, Mouse, Mouse bone marrow stromal cells (BMSCs).
-
Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells.
Rubinek T, Chesnokova V, Wolf I, Wawrowsky K, Vlotides G, Melmed S.
American Journal of Physiology. Cell Physiology 2007 Sep; 293(3):C1082.
Application：IP, WB, Mouse , Bone marrow stem cells.
-
Processing, localization, and requirement of human separase for normal anaphase progression.
Chestukhin A, Pfeffer C, Milligan S, DeCaprio JA, Pellman D.
PNAS 2003 Apr; 100(8):4574.
-
Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com