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Last updated: 2017/12/10
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ESPL1 monoclonal antibody, clone XJ11-4D7

  • Catalog # : MAB2347
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ESPL1.
  • Immunogen:
  • Recombinant fusion protein corresponding to amino acids 1866-1996 of human ESPL1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to human separase.
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunofluorescence (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In buffer containing 0.09% sodium azide
  • Storage Instruction:
  • Store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Overlay (blue) of centrosomal staining in HeLa cells. Centrosomal staining of ESPL1 (yellow), using ESPL1 monoclonal antibody, clone XJ11-4D7 (Cat # MAB2347), and gamma tubulin (green) in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 9700
  • Gene Name:
  • ESPL1
  • Gene Alias:
  • Gene Description:
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • Gene Summary:
  • Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
  • Other Designations:
  • extra spindle poles like 1,separin, separase
  • Gene Pathway
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