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Last updated: 2017/10/15
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HIF1A monoclonal antibody, clone ESEE122

  • Catalog # : MAB2342
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HIF1A.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 329-530 of human HIF1A.
  • Host:
  • Mouse
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to HIF-1 alpha.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1:100-1:5000)
    Immunofluorescence (1:100-1:5000)
    Immunohistochemistry (Frozen sections) (1:100-1:5000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In buffer containing 0.09% sodium azide
  • Storage Instruction:
  • Store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Negative control stain of human placenta (from sea level) using mouse IgG at 1 : 100. 4uM paraffin-embedded section.
  • Immunohistochemistry (Frozen sections)
  • Immunohistochemistry
  • Immunohistochemistry
  • Staining of HIF1A in hypoxia-induced human placenta using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Detection of HIF1A (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
  • Immunofluorescence
  • Immunofluorescence
  • Staining of HIF1A (red dye 568) in a cultured Raw 264.7 cell line, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
    Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
  • Immunoprecipitation
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3091
  • Gene Name:
  • HIF1A
  • Gene Alias:
  • HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
  • Gene Description:
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Gene Summary:
  • Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • ARNT interacting protein,hypoxia-inducible factor 1, alpha subunit,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1
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