HIF1A monoclonal antibody, clone ESEE122
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Negative control stain of human placenta (from sea level) using mouse IgG at 1 : 100. 4uM paraffin-embedded section.
Immunohistochemistry
Staining of HIF1A in hypoxia-induced human placenta using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
Immunofluorescence
Staining of HIF1A (red dye 568) in a cultured Raw 264.7 cell line, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
Immunofluorescence
Detection of HIF1A (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HIF1A.
Immunogen
Recombinant protein corresponding to amino acids 329-530 of human HIF1A.
Host
Mouse
Reactivity
Bovine, Dog, Human, Mouse, Rat
Specificity
This antibody is specific to HIF-1 alpha.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunocytochemistry (1:100-1:5000)
Immunofluorescence (1:100-1:5000)
Immunohistochemistry (Frozen sections) (1:100-1:5000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Negative control stain of human placenta (from sea level) using mouse IgG at 1 : 100. 4uM paraffin-embedded section.Immunohistochemistry (Frozen sections)
Immunohistochemistry
Staining of HIF1A in hypoxia-induced human placenta using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).Immunocytochemistry
Immunofluorescence
Staining of HIF1A (red dye 568) in a cultured Raw 264.7 cell line, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
Photos courtesy of Susan Alexander and Hattie Gresham, PhD.Immunofluorescence
Detection of HIF1A (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).Immunoprecipitation
-
Gene Info — HIF1A
Entrez GeneID
3091Protein Accession#
Q16665Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Are mast cells implicated in asphyxia?
Muciaccia B, Sestili C, De Grossi S, Vestri A, Cipolloni L, Cecchi R.
International Journal of Legal Medicine 2016 Jan; 130(1):153.
Application：IF, IHC-P, Human, Lung.
-
Markers of mechanical asphyxia: immunohistochemical study on autoptic lung tissues.
Cecchi R, Sestili C, Prosperini G, Cecchetto G, Vicini E, Viel G, Muciaccia B.
International Journal of Legal Medicine 2014 Jan; 128(1):117.
Application：IHC, Human, Lung.
-
Antitumor activity of an oncolytic adenoviral-CD40 ligand (CD154) transgene construct in human breast cancer cells.
Gomes EM, Rodrigues MS, Phadke AP, Butcher LD, Starling C, Chen S, Chang D, Hernandez-Alcoceba R, Newman JT, Stone MJ, Tong AW.
Clinical Cancer Research 2009 Feb; 15(4):1317.
-
Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours.
Kubo T, Sugita T, Shimose S, Matsuo T, Arihiro K, Ochi M.
The Journal of Bone and Joint Surgery. British Volume 2008 Mar; 90(3):364.
Application：IHC-P, Human, Human chondrosarcoma.
-
Differential regulation of hypoxia-induced CXCR4 triggering during B-cell development and lymphomagenesis.
Piovan E, Tosello V, Indraccolo S, Masiero M, Persano L, Esposito G, Zamarchi R, Ponzoni M, Chieco-Bianchi L, Dalla-Favera R, Amadori A.
Cancer Research 2007 Sep; 67(18):8605.
Application：IHC-P, WB-Tr, Human, Mouse, BRG cells, primary Burkitt's-like lymphomas.
-
Hypoxia at the site of abdominal aortic aneurysm rupture is not associated with increased lactate.
Choke E, Cockerill GW, Dawson J, Chung YL, Griffiths J, Wilson RW, Loftus IM, Thompson MM.
Annals of the New York Academy of Sciences 2006 Nov; 1085:306.
-
Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation.
Khan Z, Michalopoulos GK, Stolz DB.
The American Journal of Pathology 2006 Oct; 169(4):1251.
Application：IEM, IF, IHC-P, Rat, Rat livers, hepatocytes.
-
Are mast cells implicated in asphyxia?
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com