Mouse monoclonal antibody raised against partial recombinant HIF1A.
Recombinant protein corresponding to amino acids 329-530 of human HIF1A.
Bovine, Dog, Human, Mouse, Rat
This antibody is specific to HIF-1 alpha.
Immunocytochemistry (1:100-1:5000) Immunofluorescence (1:100-1:5000) Immunohistochemistry (Frozen sections) (1:100-1:5000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:5000) The optimal working dilution should be determined by the end user.
In buffer containing 0.09% sodium azide
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
ARNT interacting protein,hypoxia-inducible factor 1, alpha subunit,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1