PRKCA (phospho T497) monoclonal antibody, clone F1 (FITC)
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More Files
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Flow Cytometry
Flow cytometric analysis of NIH3T3 cells with PRKCA (phospho Thr497) monoclonal antibody, clone F1 (FITC)(Cat # MAB23384). Treated with staurosporine and unstained as negative control (blue) or treated with staurosporine (red) or with PDGF (green).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PRKCA.
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKCalpha
Host
Rabbit
Reactivity
Human
Form
Liquid
Conjugation
FITC
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (5 uL/million cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% NaN3, 0.2% BSA)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Flow cytometric analysis of NIH3T3 cells with PRKCA (phospho Thr497) monoclonal antibody, clone F1 (FITC)(Cat # MAB23384). Treated with staurosporine and unstained as negative control (blue) or treated with staurosporine (red) or with PDGF (green). -
Gene Info — PRKCA
Entrez GeneID
5578Protein Accession#
P17252Gene Name
PRKCA
Gene Alias
AAG6, MGC129900, MGC129901, PKC-alpha, PKCA, PRKACA
Gene Description
protein kinase C, alpha
Omim ID
176960Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
Other Designations
aging-associated gene 6|protein kinase C alpha type
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Interactome
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Pathway
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Disease
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