LMO2 monoclonal antibody, clone 1A9-3B11
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot Analysis of LMO2 in Ramos cell lysate using LMO2 monoclonal antibody, clone 1A9-3B11 (Cat #MAB2336).
Immunofluorescence
Immunofluorescent labeling of LMO2 (green) on human tonsil tissue using LMO2 monoclonal antibody, clone 1A9-3B11 (Cat #MAB2336).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant LMO2.
Immunogen
Recombinant protein corresponding to human LMO2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:500)
Western Blot (0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot Analysis of LMO2 in Ramos cell lysate using LMO2 monoclonal antibody, clone 1A9-3B11 (Cat #MAB2336).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunofluorescence
Immunofluorescent labeling of LMO2 (green) on human tonsil tissue using LMO2 monoclonal antibody, clone 1A9-3B11 (Cat #MAB2336).Immunoprecipitation
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Gene Info — LMO2
Entrez GeneID
4005Protein Accession#
Q86U70Gene Name
LMO2
Gene Alias
RBTN2, RBTNL1, RHOM2, TTG2
Gene Description
LIM domain only 2 (rhombotin-like 1)
Omim ID
180385Gene Ontology
HyperlinkGene Summary
LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms
Other Designations
LIM domain only 2|T-cell translocation gene 2|rhombotin 2|rhombotin-like 1
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Interactome
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Disease
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Publication Reference
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PAK5 is a potential target in myelodysplastic syndrome through interacting with LMO2 and GATA1.
Xiaoyan Pan, Dawei Liu, Minchao Ying, Gaoming Zheng, Chaoming Fan, Feng Pan, Qiang Ke.
Cellular and Molecular Biology (Noisy-le-Grand, France) 2022 Sep; 68(9):77.
Application：IF, Human, K562, THP1 cells.
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A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential for tumor growth and survival.
Nakayama K, Nakayama N, Davidson B, Sheu JJ, Jinawath N, Santillan A, Salani R, Bristow RE, Morin PJ, Kurman RJ, Wang TL, Shih IeM.
PNAS 2006 Dec; 103(49):18739.
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PAK5 is a potential target in myelodysplastic syndrome through interacting with LMO2 and GATA1.
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