SaCas9 monoclonal antibody, clone 26H10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of SaCas9 monoclonal antibody, clone 26H10 with transfected or untransfected cell lysates at 1 ug/mL. (1) 50 ug transfected Hela (2) 50 ug untransfected Hela (3) 40 ng SaCas9 monoclonal antibody
Immunofluorescence
Immunofluorescence staining of SaCas9 monoclonal antibody, clone 26H10 in Hela cells.
Immunoprecipitation
Immunoprecipitation analysis of SaCas9 monoclonal antibody, clone 26H10 with transfected or untransfected cell lysates at 1 ug/mL. (1) 50 ug transfected Hela (2) Transfected Hela cell lysate (200 ug), protein A magbeads (20 uL), Purified Antibody (10 ug) (3) Hela cell lysate (200 ug), protein A magbeads (20 uL), Purified Antibody (10 ug) (4) Transfected Hela cell lysate, protein A magbeads (20 uL), Mouse IgG (10 ug).
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant cas9 protein.
Host
Mouse
Reactivity
Staphylococcus
Form
Lyophilized
Purification
Protein A purification
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Recommend Usage
CRISPR Genome editing
ELISA (0.005-1 ug/mL)
Immunofluorescence (0.2-1 ug/mL)
Immunoprecipitation
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of SaCas9 monoclonal antibody, clone 26H10 with transfected or untransfected cell lysates at 1 ug/mL. (1) 50 ug transfected Hela (2) 50 ug untransfected Hela (3) 40 ng SaCas9 monoclonal antibodyImmunofluorescence
Immunofluorescence staining of SaCas9 monoclonal antibody, clone 26H10 in Hela cells.Immunoprecipitation
Immunoprecipitation analysis of SaCas9 monoclonal antibody, clone 26H10 with transfected or untransfected cell lysates at 1 ug/mL. (1) 50 ug transfected Hela (2) Transfected Hela cell lysate (200 ug), protein A magbeads (20 uL), Purified Antibody (10 ug) (3) Hela cell lysate (200 ug), protein A magbeads (20 uL), Purified Antibody (10 ug) (4) Transfected Hela cell lysate, protein A magbeads (20 uL), Mouse IgG (10 ug).CRISPR Genomic Editing
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