CRISPR-Cas9 monoclonal antibody, clone 6F7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of CRISPR-Cas9 monoclonal antibody, clone 6F7 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa. Labeling is absent in the untransfected lystae (-).
Immunofluorescence
Immunoflourescence staining of CRISPR-Cas9 monoclonal antibody, clone 6F7 transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas 9 protein (green) and the DNA stained blue with DAPI. The yellow label is the overlapping of the CRISPR-Cas9 monoclonal antibody and the Cas9-GFP construct.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant cas9 protein.
Host
Mouse
Reactivity
Staphylococcus
Form
Liquid
Purification
Protein G purification
Recommend Usage
CRISPR Genome editing
Immunofluorescence (1:500)
Immunohistochemistry (1:1000)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% Glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of CRISPR-Cas9 monoclonal antibody, clone 6F7 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa. Labeling is absent in the untransfected lystae (-).Immunofluorescence
Immunoflourescence staining of CRISPR-Cas9 monoclonal antibody, clone 6F7 transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas 9 protein (green) and the DNA stained blue with DAPI. The yellow label is the overlapping of the CRISPR-Cas9 monoclonal antibody and the Cas9-GFP construct.CRISPR Genomic Editing
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