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Last updated: 2022/7/3

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SaCas9 Monoclonal Antibody, clone 11C12 

  • Catalog # : MAB23324
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Streptococcus aureus Cas9.
  • Immunogen:
  • Recombinant protein corresponding to Staphylococcus aureus Cas9.
  • Host:
  • Mouse
  • Reactivity:
  • Streptococcus
  • Specificity:
  • This antibody reacts to Staphylococcus aureus Cas9.
  • Form:
  • Lyophilized
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG2b Kappa
  • Recommend Usage:
  • ELISA (0.005-1 ug/mL)
    Immunofluorescence (0.2-1 ug/mL)
    Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH7.4(0.02% sodium azide).
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
  • Storage Instruction:
  • Store at -20°C.
    Upon reconstitution, it can be stored for up to 12 months at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot of Hela transfected with PX458 (SpCas9(BB)-2A-GFP) or untransfected cell lysates with SaCas9 Monoclonal Antibody, clone 11C12.
    Lane 1: 50 ug cell lysate of Hela transfected with SpCas9(BB)-2A-GFP
    Lane 2: 50 ug untransfected Hela cell lysate
    Lane 3: 40 ug SpCas9 recombinant protein
    Primary Antibody:
    SaCas9 Monoclonal Antibody, clone 11C12 1 ug/mL
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mL
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of SaCas9 Monoclonal Antibody, clone 11C12 with recombinant Streptococcus aureus CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 ug/mL. SaCas9 Monoclonal Antibody, clone 11C12 dilution start from 1000 ng/mL, EC50= 3.75 ng/mL.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
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