SaCas9 Monoclonal Antibody, clone 11C12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot of Hela transfected with PX458 (SpCas9(BB)-2A-GFP) or untransfected cell lysates with SaCas9 Monoclonal Antibody, clone 11C12.
Lane 1: 50 ug cell lysate of Hela transfected with SpCas9(BB)-2A-GFP
Lane 2: 50 ug untransfected Hela cell lysate
Lane 3: 40 ug SpCas9 recombinant protein
Primary Antibody:
SaCas9 Monoclonal Antibody, clone 11C12 1 ug/mL
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mL
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Specification
Product Description
Mouse monoclonal antibody raised against Streptococcus aureus Cas9.
Immunogen
Recombinant protein corresponding to Staphylococcus aureus Cas9.
Host
Mouse
Reactivity
Streptococcus
Specificity
This antibody reacts to Staphylococcus aureus Cas9.
Form
Lyophilized
Purification
Protein A affinity purification
Isotype
IgG2b Kappa
Recommend Usage
ELISA (0.005-1 ug/mL)
Immunofluorescence (0.2-1 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4(0.02% sodium azide).
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.Storage Instruction
Store at -20°C.
Upon reconstitution, it can be stored for up to 12 months at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot of Hela transfected with PX458 (SpCas9(BB)-2A-GFP) or untransfected cell lysates with SaCas9 Monoclonal Antibody, clone 11C12.
Lane 1: 50 ug cell lysate of Hela transfected with SpCas9(BB)-2A-GFP
Lane 2: 50 ug untransfected Hela cell lysate
Lane 3: 40 ug SpCas9 recombinant protein
Primary Antibody:
SaCas9 Monoclonal Antibody, clone 11C12 1 ug/mL
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mLEnzyme-linked Immunoabsorbent Assay
ELISA binding of SaCas9 Monoclonal Antibody, clone 11C12 with recombinant Streptococcus aureus CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 ug/mL. SaCas9 Monoclonal Antibody, clone 11C12 dilution start from 1000 ng/mL, EC50= 3.75 ng/mL.
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