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Last updated: 2022/7/3

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SpCas9 Monoclonal Antibody, clone 4A1 

  • Catalog # : MAB23322
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Streptococcus pyogenes Cas9.
  • Immunogen:
  • Recombinant protein corresponding to Staphylococcus pyogenes Cas9.
  • Host:
  • Mouse
  • Reactivity:
  • Streptococcus pyogenes
  • Specificity:
  • This antibody reacts to spCas9.
  • Form:
  • Lyophilized
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Standard Curve

    QC Testing of MAB23322
    The standard curve is for the purpose of illustration only and should not be used to calculate unknowns.
    A standard curve should be generated each time the assay is performed.
    In this ELISA assay, SpCas9 Monoclonal Antibody was labeled with Biotin. The sensitivity is <1 ng/mL and the detection range is 0-10 ng/mL.
  • Recommend Usage:
  • ELISA (0.01-1 ug/mL)
    Immunofluorescence (0.2-1 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH7.4 (0.02% sodium azide).
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
  • Storage Instruction:
  • Store at -20°C.
    Upon reconstitution, it can be stored for up to 12 months at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Monoclonal Antibody, clone 4A1.
    Lane 1: 50 ug cell lysate of HEK293 transfected with PX458
    Lane 2: 50 ug untransfected HEK293 cell lysate
    Lane 3: 40 ug SpCas9 recombinant protein
    Primary Antibody:
    SpCas9 Monoclonal Antibody, clone 4A1 1 ug/mL
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mL
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • SPy_1046
  • Gene Alias:
  • -
  • Gene Description:
  • hypothetical protein
  • Other Designations:
  • -
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