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Last updated: 2022/8/14

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CRISPR-SaCas9 Monoclonal Antibody, clone 6F7 

  • Catalog # : MAB23321
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Streptococcus aureus Cas9.
  • Immunogen:
  • Recombinant protein corresponding to C-terminus of Staphylococcus aureus Cas9.
  • Host:
  • Mouse
  • Reactivity:
  • Streptococcus
  • Specificity:
  • This antibody reacts to Staphylococcus aureus Cas9.
    No reactivity toward the Streptococcus pyogenes Cas9.
  • Form:
  • Liquid
  • Purification:
  • Protein G affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunofluorescence (1:500-1:5000)
    Immunohistochemistry (1:1000-1:5000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 5 mM NaN3)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with CRISPR-SaCas9 Monoclonal Antibody, clone 6F7 (1:500, red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
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