CRISPR-SaCas9 Monoclonal Antibody, clone 6F7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).
Immunofluorescence
Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with CRISPR-SaCas9 Monoclonal Antibody, clone 6F7 (1:500, red).
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Specification
Product Description
Mouse monoclonal antibody raised against Streptococcus aureus Cas9.
Immunogen
Recombinant protein corresponding to C-terminus of Staphylococcus aureus Cas9.
Host
Mouse
Reactivity
Streptococcus
Specificity
This antibody reacts to Staphylococcus aureus Cas9.
No reactivity toward the Streptococcus pyogenes Cas9.Form
Liquid
Purification
Protein G affinity purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1:500-1:5000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 5 mM NaN3)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).Immunofluorescence
Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with CRISPR-SaCas9 Monoclonal Antibody, clone 6F7 (1:500, red).
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