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Last updated: 2016/12/4
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NES monoclonal antibody, clone 10C2

  • Catalog # : MAB2332
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NES.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1464-1614 of human NES.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to human nestin.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry (1:50)
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Western Blot (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In buffer containing 0.09% sodium azide
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Detection of human NES using NES monoclonal antibody, clone 10C2 (Cat # MAB2332). Lane 1 : 5 ug total rat brain protein. Lane 2 : 5 ug human CNS progenitor cell protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Visualization of NES in PC-3 cells using NES monoclonal antibody, clone 10C2 (Cat # MAB2332). Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • NES
  • Gene Alias:
  • FLJ21841,Nbla00170
  • Gene Description:
  • nestin
  • Gene Summary:
  • Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000032198
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