Kaposi's sarcoma-associated herpesvirus LANA2 monoclonal antibody, clone CM-A807
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of LANA2 in infected lysates (30 ug) using 2 ug/mL of LANA2 monoclonal antibody, clone CM-A807 (Cat # MAB2328).
ECL detection 1.5 minutes.
Lane 1: BCBL-1 (KSHV+).
Lane 2 : BC-1 (KSHV+/EBV+).
Lane 3 : BCP-1 (KSHV+).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant Kaposi's sarcoma-associated herpesvirus LANA2.
Immunogen
Recombinant fusion protein corresponding to Kaposi's sarcoma-associated herpesvirusLANA2.
Host
Mouse
Theoretical MW (kDa)
66-70
Reactivity
Human, Mouse
Specificity
This antibody is specific to KSHV LANA2 (residues 143-310 epitope recognition). By Western Blot, this antibody recognizes a viral protein in KSHV infected B-cells (~66-70 KDa).
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of LANA2 in infected lysates (30 ug) using 2 ug/mL of LANA2 monoclonal antibody, clone CM-A807 (Cat # MAB2328).
ECL detection 1.5 minutes.
Lane 1: BCBL-1 (KSHV+).
Lane 2 : BC-1 (KSHV+/EBV+).
Lane 3 : BCP-1 (KSHV+).Immunofluorescence
Immunoprecipitation
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Publication Reference
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Kaposi's sarcoma-associated herpesvirus viral IFN regulatory factor 3 stabilizes hypoxia-inducible factor-1 alpha to induce vascular endothelial growth factor expression.
Shin YC, Joo CH, Gack MU, Lee HR, Jung JU.
Cancer Research 2008 Mar; 68(6):1751.
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Kaposi's sarcoma-associated herpesvirus LANA2 is a B-cell-specific latent viral protein that inhibits p53.
Rivas C, Thlick AE, Parravicini C, Moore PS, Chang Y.
Journal of Virology 2001 Jan; 75(1):429.
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Kaposi's sarcoma-associated herpesvirus viral IFN regulatory factor 3 stabilizes hypoxia-inducible factor-1 alpha to induce vascular endothelial growth factor expression.
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