Mouse monoclonal antibody raised against partial recombinant Prdm1.
Recombinant protein corresponding to amino acids 199-409 of mouse Prdm1.
Theoretical MW (kDa):
Human, Mouse, Pig
This antibody is specific to the Blimp-1. Recognizes a band ~98 KDa and may recognize one ~80 KDa (the beta form).
Flow Cytometry (8 ug/mL, 2*10^6/mL cells) Immunocytochemistry (1:50-1:100) Immunofluorescence (1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide)
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Detection of Prdm1 in murine plasmacytoma cell lysate (P3X) using Prdm1 monoclonal antibody, clone 3H2-E8 (Cat # MAB2327). Murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.
Double IF for Prdm1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10 um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.