25-OH Vitamin D3 Rabbit/Human chimeric monoclonal antibody, clone RMH04
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of 25(OH) Vitamin D Standard Curve: ELISA plate was coated with 100 ng capture antibody RMH04 at concentration of 1ug/mL. A serial dilution from 150 ng/mL was performed using 25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 monoclonal antibody, clone RM428 was used as a detection antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against 25-OH Vitamin D3/RM3,D3/RMH04.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against 25-OH Vitamin D3 BSA conjugate.
Specificity
This antibody reacts to 25-OH Vitamin D3. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (50 ng/well-200 ng/well)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of 25(OH) Vitamin D Standard Curve: ELISA plate was coated with 100 ng capture antibody RMH04 at concentration of 1ug/mL. A serial dilution from 150 ng/mL was performed using 25-OH Vitamin D3/RM3 or 25-OH Vitamin D3/ RMH04 monoclonal antibody, clone RM428 was used as a detection antibody.
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