Histone H4 (symmetric dimethyl R3) monoclonal antibody, clone RM459
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic symmetric dimethyl peptide corresponding to residues surroundin Arg3 of human histone H4.
Reactivity
Human
Specificity
This antibody reacts to Histone H4 symmetric dimethylated at Arginine 3 (R3me2s). No cross reactivity with unmodified Arginine 3, monomethylated or asymmetric dimethylated Arginine 3, or other methylated Arginine in Histone H4.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (0.1 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: acid extracts of K-562 cell lysate, Lane 2: histone H4 recombinant protein using HIST4H4 (symmetric dimethyl R3) monoclonal antibody, clone RM459 (Cat # MAB23243) under 0.1 ug/mL working concentration.
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