Histone H4 (monomethyl K12) monoclonal antibody, clone RM458
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: histone H4 recombinant protein, Lane 2: acid extracts of HeLa cell lysate using HIST4H4 (monomethyl K12) monoclonal antibody, clone RM458 (Cat # MAB23242) under 0.2 ug/mL working concentration.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H4 (monomethyl K12) monoclonal antibody, clone RM458 (Cat# MAB23242) specifically reacts to Histone H4 monomethylated at Lysine 12 (K12me1). No cross reactivity with non-modified Lysine 12, or other monomethylations in histone H4.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic monomethyl peptide corresponding to residues surrounding Arg12 of human histone H4.
Reactivity
Human
Specificity
This antibody reacts to Histone H4 monomethylated at Lysine 12 (K12me1). No cross reactivity with non-modified Lysine 12, or other monomethylations in histone H4.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (0.01 ug/mL-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: histone H4 recombinant protein, Lane 2: acid extracts of HeLa cell lysate using HIST4H4 (monomethyl K12) monoclonal antibody, clone RM458 (Cat # MAB23242) under 0.2 ug/mL working concentration.
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