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Last updated: 2022/5/22

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HIST4H4 (monomethyl K5) monoclonal antibody, clone RM457 

  • Catalog # : MAB23241
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic monomethyl peptide of human HIST4H4 (monomethyl K5).
  • Immunogen:
  • A synthetic monomethyl peptide corresponding to residues surrounding R5 of HIST4H4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to HIST4H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations histone H4.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (0.1 ug/mL-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: histone H4 recombinant protein, Lane 2: acid extracts of HeLa cell lysate using HIST4H4 (monomethyl K5) monoclonal antibody, clone RM457 (Cat # MAB23241) under 0.2 ug/mL working concentration.
  • Application Image
  • Gene Information
  • Gene Name:
  • HIST4H4
  • Gene Alias:
  • H4/p,MGC24116
  • Gene Description:
  • histone cluster 4, H4
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq
  • Other Designations:
  • histone 4, H4,histone H4
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