Histone H4 (monomethyl K5) monoclonal antibody, clone RM457
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: histone H4 recombinant protein, Lane 2: acid extracts of HeLa cell lysate using HIST4H4 (monomethyl K5) monoclonal antibody, clone RM457 (Cat # MAB23241) under 0.2 ug/mL working concentration.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H4 (monomethyl K5) monoclonal antibody, clone RM457 (Cat# MAB23241) specifically reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in histone H4.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic monomethyl peptide corresponding to residues surrounding Arg5 of human histone H4.
Reactivity
Human
Specificity
This antibody reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations histone H4.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (0.1 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: histone H4 recombinant protein, Lane 2: acid extracts of HeLa cell lysate using HIST4H4 (monomethyl K5) monoclonal antibody, clone RM457 (Cat # MAB23241) under 0.2 ug/mL working concentration.
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