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EIF4E (phospho S209) monoclonal antibody, clone RM452 

  • Catalog # : MAB23236
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human EIF4E.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S209 of human EIF4E.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to human EIF4E only when phosphorylated at Ser209. There is no cross-reactivity to EIF4E that is not phosphorylated. This antibody may also react to mouse or rat Phospho-EIF4E (Ser209) as predicted by immunogen homology.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of MCF-7 cell lysates with EIF4E (phospho S209) monoclonal antibody, clone RM452 (Cat # MAB23236) at a 1:1000 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1977
  • Gene Name:
  • EIF4E
  • Gene Alias:
  • CBP,EIF4E1,EIF4EL1,EIF4F,MGC111573
  • Gene Description:
  • eukaryotic translation initiation factor 4E
  • Gene Summary:
  • All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
  • Other Designations:
  • eIF-4F 25 kDa subunit,eukaryotic translation initiation factor 4E-like 1,mRNA cap-binding protein
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