Histone H2AX (acetyl K5) monoclonal antibody, clone RM445
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: H2A recombinant protein, Lane 2: acid extracts of HeLa cell lysate using H2AFX (acetyl K5) monoclonal antibody, clone RM445 (Cat # MAB23230) under 0.04 ug/mL working concentration.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H2AX (acetyl K5) monoclonal antibody, clone RM445 (Cat# MAB23230) specifically reacts to Histone H2AX acetylated at Lysine 5 (K5ac). No or limited cross reactivity with acetylated Lysine 9 (K9ac), Lysine 36 (K36ac) in Histone H2AX, Lysine 4 (K4ac), Lysine 7 (K7ac), Lysine 11 (K11ac), Lysine 13 (K13ac) in Histone H2AZ, or non-modified Asparagine 19 (N19), Lysine 36 (K36) in Histone H2AX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H2AX.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic acetyl peptide corresponding to residues surrounding Lys5 of human histone H2AX.
Reactivity
Human
Specificity
This antibody reacts to histone H2AX acetylated at Lysine 5 (K5ac). No cross reactivity with non-modified Lysine 5 or other acetylated Lysines in histone H2A.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (0.04 ug/mL-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: H2A recombinant protein, Lane 2: acid extracts of HeLa cell lysate using H2AFX (acetyl K5) monoclonal antibody, clone RM445 (Cat # MAB23230) under 0.04 ug/mL working concentration. -
Gene Info — H2AFX
Entrez GeneID
3014Gene Name
H2AFX
Gene Alias
H2A.X, H2A/X, H2AX
Gene Description
H2A histone family, member X
Omim ID
601772Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
Other Designations
H2AX histone
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Interactome
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Pathway
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Disease
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