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Last updated: 2022/8/14

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Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 

  • Catalog # : MAB23229
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human Detyrosinated alpha Tubulin.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of detyrosinated alpha-Tubulin.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to Detyrosinated alpha Tubulin a PTM of α-tubulin where the C-terminal tyrosine residue has been cleaved.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:100-1:200)
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1:HeLa, Lane 2:U-2 OS, Lane 3:CHL-1 cell lysates with Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 (Cat # MAB23229) at a 1:1000 dilution.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of CHL-1 using Detyrosinated alpha Tubulin monoclonal antibody, clone RM444 (Cat # MAB23229) at a 1:200 dilution (pink), anti-alpha-Tubulin (green) antibody, and DAPI (blue). Bar: 20 um.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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