Mouse monoclonal antibody raised against native luciferase.
Native purified luciferase protein from firefly, Photinus pyralis.
Theoretical MW (kDa):
This antibody recognizes a band at ~61 KDa on Western Blot. specific to luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time.
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In buffer containing 0.09% sodium azide
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Detection of luciferase expression in CD34+ cells by immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with Luciferase monoclonal antibody, clone Luci 21 1-107 (Cat # MAB2322). Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, x 40. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102 (10) : 3478-3482, 2003.