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Luciferase monoclonal antibody, clone Luci 21 1-107

  • Catalog # : MAB2322
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native luciferase.
  • Immunogen:
  • Native purified luciferase protein from firefly, Photinus pyralis.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 61
  • Reactivity:
  • Firefly
  • Specificity:
  • This antibody recognizes a band at ~61 KDa on Western Blot. specific to luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In buffer containing 0.09% sodium azide
  • Storage Instruction:
  • Store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunofluorescence
  • Immunofluorescence
  • Detection of luciferase expression in CD34+ cells by immunohistochemistry.
    Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with Luciferase monoclonal antibody, clone Luci 21 1-107 (Cat # MAB2322).
    Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue.
    Nontransduced, cultured CD34+ cells were used as a negative control.
    Original magnification, x 40.
    Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102 (10) : 3478-3482, 2003.
  • Application Image
  • Western Blot
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