Luciferase monoclonal antibody, clone Luci 21 1-107
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More Files
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Immunofluorescence
Detection of luciferase expression in CD34+ cells by immunohistochemistry.
Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with Luciferase monoclonal antibody, clone Luci 21 1-107 (Cat # MAB2322).
Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue.
Nontransduced, cultured CD34+ cells were used as a negative control.
Original magnification, x 40.
Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102 (10) : 3478-3482, 2003.
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Specification
Product Description
Mouse monoclonal antibody raised against native luciferase.
Immunogen
Native purified luciferase protein from firefly, Photinus pyralis.
Host
Mouse
Theoretical MW (kDa)
61
Reactivity
Firefly
Specificity
This antibody recognizes a band at ~61 KDa on Western Blot. specific to luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Detection of luciferase expression in CD34+ cells by immunohistochemistry.
Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with Luciferase monoclonal antibody, clone Luci 21 1-107 (Cat # MAB2322).
Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue.
Nontransduced, cultured CD34+ cells were used as a negative control.
Original magnification, x 40.
Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102 (10) : 3478-3482, 2003. -
Publication Reference
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Inducible alterations of glutathione levels in adult dopaminergic midbrain neurons result in nigrostriatal degeneration.
Chinta SJ, Kumar MJ, Hsu M, Rajagopalan S, Kaur D, Rane A, Nicholls DG, Choi J, Andersen JK.
Journal of Neuroscience 2007 Dec; 27(51):13997.
Application：IHC, Mouse, Mouse brain.
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Imaging of mesenchymal stem cell transplant by bioluminescence and PET.
Love Z, Wang F, Dennis J, Awadallah A, Salem N, Lin Y, Weisenberger A, Majewski S, Gerson S, Lee Z.
Journal of Nuclear Medicine 2007 Dec; 48(12):2011.
Application：Flow Cyt, Human, Human mesenchymal stem cells, K562 cells.
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p53 short peptide (p53pep164) regulates lipopolysaccharide-induced tumor necrosis factor-alpha factor/cytokine expression.
Tang X, Molina M, Amar S.
Cancer Research 2007 Feb; 67(3):1308.
Application：WB-Tr, Human, H1299 cells.
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The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis.
Akpovi CD, Yoon SR, Vitale ML, Pelletier RM.
Journal of Lipid Research 2006 Oct; 47(10):2233.
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Inducible alterations of glutathione levels in adult dopaminergic midbrain neurons result in nigrostriatal degeneration.
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